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Perit Dial Int 28(3): 277-282 2008
© 2008 International Society for Peritoneal Dialysis
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ORIGINAL ARTICLES

3,4-DGE IN PERITONEAL DIALYSIS FLUIDS CANNOT BE FOUND IN PLASMA AFTER INFUSION INTO THE PERITONEAL CAVITY

Martin Erixon1,2, Anders Wieslander1, Torbjörn Lindén1, Ola Carlsson1, Jan Åke Jönsson2, Ole Simonsen3 and Per Kjellstrand1

1 Gambro AB; 2 Analytical Chemistry, University of Lund; 3 University Hospital of Lund, Lund, Sweden

Correspondence to: M. Erixon, Gambro AB, Box 10101, S-220 10, Lund, Sweden.
martin.erixon{at}gambro.com

{diamondsuit} Objective: Glucose degradation products (GDPs) are important in the outcome of peritoneal dialysis (PD) treatment. 3,4-dideoxyglucosone-3-ene (3,4-DGE) is the most cytotoxic GDP found in conventionally manufactured fluids and may, in addition, be recruited from 3-deoxyglucosone (3-DG). It is not known what happens with those GDPs in patients during PD. The aim of this study was to investigate if the 3,4-DGE and 3-DG in PD fluids can be found in plasma during treatment.

{diamondsuit} Design: PD patients were dialyzed with a conventional PD fluid containing 43 µmol/L 3,4-DGE and 281 µmol/L 3-DG. Parallel experiments were performed in rats as well as in vitro with human plasma. The rats were dialyzed with a PD fluid containing 100 µmol/L 3,4-DGE and 200 µmol/L 3-DG.

{diamondsuit} Results: The concentration of 3,4-DGE in the peritoneum decreased at a much higher rate than 3-DG during the dwell. 3,4-DGE was not, however, detected in the plasma of patients or rats during dialysis. The concentration of 3-DG in plasma peaked shortly after infusion of the fluid to the peritoneal cavity. The concentration of 3,4-DGE during experimental incubation in plasma decreased rapidly, while the concentration of 3-DG decreased only 10% as rapidly or less.

{diamondsuit} Conclusion: 3,4-DGE could not be detected in plasma from either PD patients or rats during dialysis. This is presumably due to its high reactivity. 3-DG may, on the other hand, pass through the membrane and be detected in the blood.

KEY WORDS: PD fluid; glucose degradation products (GDPs); 3,4-DGE; 3-DG; plasma; advanced glycation end products.

Received 11 July 2007; accepted 21 December 2007.






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