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Perit Dial Int 27(6): 654-662 2007
© 2007 International Society for Peritoneal Dialysis
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Bench Science

THE EXPRESSION PATTERNS OF PERITONEAL DEFENSINS

Alexander Grupp1,2, Martin Kimmel1, Peter Fritz3, Bernd Voggenreiter4, Hartmut Stöltzing4, Ulrich Kuhlmann1, Eduard F. Stange2, Thomas Mettang5, Klaus Fellermann2 and Dominik M. Alscher1

Division of General Internal Medicine and Nephrology1 and Division of Gastroenterology, Hepatology and Endocrinology,2 Department of Internal Medicine; Division of Pathology,3 Department of Diagnostic Medicine; Division of General Surgery,4 Department of Surgery, Robert-Bosch Krankenhaus, Stuttgart; Fachbereich Nephrologie,5 Deutsche Klinik für Diagnostik, Wiesbaden, Germany

Correspondence to: D.M. Alscher, Robert-Bosch Krankenhaus, Auerbachstrasse 110, D-70376 Stuttgart, Germany. dominik.alscher{at}rbk.de

{diamondsuit} Background: Local defense mechanisms are important for the integrity of the peritoneum, but few details are known about the expression patterns of antimicrobial proteins such as human defensin in normal and damaged peritoneum.

{diamondsuit} Methods: Part A: The expression of different defensins in normal (n = 12), inflamed (n = 5), and metastatic peritoneum (n = 4) and in cultured human peritoneal mesothelial cells was analyzed using mRNA and immunohistochemistry. Part B: Using immunohistochemistry the expression of different defensins was analyzed in different subgroups: healthy controls (n = 25), patients with chronic appendicitis (n = 25) or acute appendicitis (n = 10), and end-stage renal disease patients (n = 25, with 15 on peritoneal dialysis).

{diamondsuit} Results: Part A: Human neutrophil peptides (HNP) 1 and 3 and human ß-defensins (HBD) 1 to 3 mRNA were detected in peritoneal specimens. In addition, HNP1,3, HBD1, HBD2, and HBD3 proteins were detected using immunohistochemistry. Part B: HBD1 showed a constitutive expression in mesothelium, while HBD2 and HNP1,3 were associated with inflammation. Decreased expressions of HNP1,3 were observed in end-stage renal disease patients and in patients on peritoneal dialysis.

{diamondsuit} Conclusions: For the first time, the expression patterns of defensins in normal and damaged peritoneum have been described. The reduced expression of some defensins in end-stage renal disease is of potential clinical interest against the background of the frequent infective complications seen in peritoneal dialysis.

KEY WORDS: Innate immunity; defensins; peritonitis; infective complications; local defense; mesothelium.

Received 22 March 2007; accepted 17 July 2007.






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