PDI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Perit Dial Int 23(1): 23-32 2003
© 2003 International Society for Peritoneal Dialysis
This Article
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruiz, M.
Right arrow Articles by Bellmunt, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruiz, M.
Right arrow Articles by Bellmunt, M.
Peritoneal Dialysis International, Vol 23, Issue 1, 23-32
Copyright © 2003 by International Society for Peritoneal Dialysis

Articles

Chemical and immunological characterization of oxidative nonenzymatic protein modifications in dialysis fluids

MC Ruiz, M Portero-Otin, R Pamplona, Requena JR, J Prat, MA Lafarga, M Borras, and MJ Bellmunt

Metabolic Pathophysiology Research Group, Department of Basic Medical Sciences, University of Lleida, Lleida, Spain.

BACKGROUND: Glucose degradation products (GDP) in dialysis fluids may induce nonenzymatic protein modifications, the chemical nature and biological properties of which should be better defined. AIMS: To characterize nonenzymatic protein modifications present in glucose-based peritoneal dialysis fluids (PDF) and to evaluate the relationship between concentrations of GDP and the derived nonenzymatic modifications, and the potential of PDF for generating these modifications in vitro. METHODS: The presence, distribution, and content of several nonenzymatic protein modifications in PDF were evaluated by immunological methods, by HPLC, and by gas chromatography-mass spectrometry (GC/MS). Peritoneal dialysis fluid-induced oxidative stress in cells was evaluated by flow cytometry. The potential of PDF for generating oxidative and glycoxidative modifications was examined by immunological and cross-linking analyses. RESULTS: The albumin present in PDF is modified by carboxymethyllysine (CML). GC/MS analyses of PDF proteins confirmed the presence of CML and demonstrated the occurrence of carboxyethyllysine, malondialdehyde lysine, and oxidation-derived semialdehydes. Furthermore, their concentrations in PDF proteins were significantly higher than those in plasma proteins (in all cases, p < 0.02). The concentration of pyrraline, a non-oxidative advanced glycation end-product, increased with dwell time up to 6 hours (p < 0.03). The PDF induced cellular free-radical production, which was partially inhibited by the Maillard reaction inhibitor aminoguanidine (p < 0.001).The potential to generate oxidative and glycoxidative modifications demonstrated an inverse relationship with dwell time (p < 0.05).The PDF was able to induce collagen cross-linking in a close relationship with GDP concentration. CONCLUSIONS: (1) PDF contains non-oxidative and several oxidative nonenzymatic protein modifications in higher concentrations than plasma. (2) Peritoneal dialysis fluid induces oxidative stress in vitro, which can be partially inhibited by aminoguanidine. (3) These properties are directly related to GDP concentration. (4) Peritoneal dialysis fluid is able to generate glycoxidative and oxidative damage to proteins in vitro in a dwell-time dependent fashion.




This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
S. Latcha, S. Hong, N. Gibbons, N. Kohn, and J. Mattana
Relationship between dialysate oxidized protein and peritoneal membrane transport properties in patients on peritoneal dialysis
Nephrol. Dial. Transplant., October 1, 2008; 23(10): 3295 - 3301.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Agalou, N. Ahmed, R. Babaei-Jadidi, A. Dawnay, and P. J. Thornalley
Profound Mishandling of Protein Glycation Degradation Products in Uremia and Dialysis
J. Am. Soc. Nephrol., May 1, 2005; 16(5): 1471 - 1485.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Multimed Inc. logo
Copyright © 2003 by Multimed Inc.